Flora

289 native species have been recorded in the Park.
The Western end of Anstey Hill R. P. is dominated by Pink gums (Eucalyptus fasciculosa), while the Eastern end is dominated by Long Leaved box (E. goniocalyx), an association that has rarely been preserved.
Many plants are now rare on the Adelaide Plains including: Button Daisy (Calocephalus cireus), Pussy Tails (Ptilotus erubescens), Needle Bush (Hakea rugosa), Silky Guinea Flower (Hibertia secricea) and Black Fapier Sedge (Lepidosperma calihoides).
Steeper slopes support native pine (Callitris preissii), Drooping Sheoks (Allocasuarina verticullata) and  Pink gums. Kangaroo thorn (Acacia paradoxa), Black boy (Xanthorrhea quadrangulata), Hop bush (Dodonea viscosa) and Tea tree (Leptospermum myrsinoides) make up most of the understorey species. Spring heralds a fabulous display of orchids (30 species) including the Spider orchid (Caladenia dilatata) and Wax lip orchid (Glossidia major).
 
 

Green Comb Spider-Orchid (Caladenia dilatata)
 

Veined Spider-Orchid (Caladenia reticulata)
 

Donkey Orchid (Diuris longifolia)

 

Waxlip Orchid (Glossidia major)

 

Rabbit Orchid (leptoceras menziesii)

 

Eastern Section

The eastern section of the park is dominated by E. goniocalyx and E. fasiculosa. The understorey consists of densely vegetated Acacia pycnantha (Golden Wattle) and Acacia paradoxa (Kangaroo Thorn) with less frequent Exocarpus cupressiformes (Native Cherry). The ground cover mainly consists of Hibbertia riparia (Erect Guinea Flower), Hibbertia exutiacies (Guinea Flower), Xanthorrhoea semiplana (yacca), Scaevola linearis (Rough Fan Flower), Scaevola albida (Hand Fan Flower) and Olearia ramulosa (Twiggy Daisy Bush).

North Eastern Section

In the north eastern section Eucalyptus viminalis (Manna Gum) becomes more dominant and shares the overstorey with Pink Gum and Long Leaved Box. The understorey consists of Hakea rostrata (Beaked Hakea), Golden Wattle, Kangaroo Thorn and Acacia retinodes (Wirilda).
Along the Water Gully creekline there are stands of Eucalyptus camaldulensis (River Red Gum) which forms the overstorey. The understorey along the creek line is weed infested consisting mainly of Rubus ultnnifolius (Blackberry), Olea europea (Olive Tree), Rosa rubiginosa (Sweet briar), Rosa banana (Dog Rose), Lathyrus tingitanus (Tangier Pea) and Zantedeschia aethiopica (Arum Lily). Some native understorey persists including Bursaria spinosa (Christmas Bush), Dodonea spp and Kangaroo Thorn.

Western section

The fire scar on the western hills face consists of regenerating Callitns preisii (Native Pine), Allocasuarina verticillata (Drooping Sheoak), Allocasuarina muelleriana (Slaty Sheoak), Hakea carinata, Hakea rostrata (Beaked Hakea), Leptospennum lanigerum (Silky Tea Tree), Leptospennum myrsinoides (Heath Tea Tree), Golden Wattle, Kangaroo Thorn, Calytrix tetragona (Common Fringe Myrtle), Hibbertia spp and Pink Gum.

Adjacent to Perseverance Road exists a stand of Eucalyptus luecoxylon (South Australian Blue Gum). The majority of the understorey has been cleared with some native grass species persisting eg, Themeda trianda (Kangaroo Grass), Chloris truncata (Windmill Grass) and Aristida behriana (Bush Spear Grass).

The main association on the western section consists of Native Pine, Drooping Sheoak and Pink Gum. The understorey consists of sclerophyllous shrubs which include Slaty Sheoak, Hakea carinata (Hakea), Kangaroo Thorn, Common Fringe myrtle, Beaked Hakea, Heath Tea Tree, Silky Tea Trees Twiggy Daisy Bush, Guinea Flower, Erect Guinea Flower and Yacca's.